Tree-frog hot spots in the Amazon have been established over tens of millions of years, say scientists.
To explain why some areas have greater species richness, experts analysed the distribution of 360 tree-frog species.
They found that the most diverse sites were established over 60 million years ago and more recently colonised areas had fewer unique species.
Researchers suggest this is evidence that damaged rainforest could take millions of years to recover.
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